Table of contents

Dataset

Here we demonstrate the usage of methrix to orchestrate WGBS analysis using best practices to our knowledge. Methrix provides set of function which allows easy importing of various flavors of bedgraphs generated by methylation callers, and many downstream analysis to be performed on large matrices.

The example data analysis presented here uses the following publicly available whole genome bisulfite sequencing data from GEO: GSE90553

Ziller MJ, Ortega JA, Quinlan KA, Santos DP et al. Dissecting the Functional Consequences of De Novo DNA Methylation Dynamics in Human Motor Neuron Differentiation and Physiology. Cell Stem Cell 2018 Apr 5;22(4):559-574.e9. PMID: 29551301